Control:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2
Purification:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2
Recombinant:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2
Electron Microscopy:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Vector Laboratories FL-1211-2 Peanut Agglutinin (PNA), Fluorescein Vector Laboratories FL-1071 Vicia Villosa Lectin (VVL, VVA), Fluorescein Vector Laboratories FL-1231-2 Critical commercial assays Autophagy Assay Kit Sigma-Aldrich MAK138 Cell Proliferation ELISA, BrdU Roche 11647229001 Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ThermoFisher Scientific V13242 Fluorometric ATP assay kit Abcam ab83355 HIF-1 Alpha ELISA Kit InvitrogenTM EHIF1A L-Lactate Assay Kit Abcam ab65331 RNeasy Plus Mini Kit Qiagen 74134 TAAB 812 (Epon) Premix Kit Medium TAAB T031 Deposited data Glycomics data [Database]: GlycoPost (https://glycopost.glycosmos.org/) GPST000517.0 Metabolomics data [Database]: Metabolomics Workbench (https://www.metabolomicsworkbench.org) ST003639 Phosphoproteomics data [Database]: PRIDE (https://www.ebi.ac.uk/pride/) PXD045777 Transcriptomics data [Database]: GEO (https://www.ncbi.nlm.nih.gov/geo/) GSE284635 Experimental models: Cell lines Human BLCA cell line 5637 (white male patient) ATCC HTB-9TM Human BLCA cell line HT1197 (white male patient) ATCC CRL-1473 TM (Continued on next page) iScience 28, 111758, February 21, 2025 e2
|
Vector Laboratories is a verified supplier 
Vector Laboratories manufactures this product 